ALG11 YNL048W alpha-1,2-mannosyltransferase activity protein amino acid glycosylation endoplasmic reticulum* Specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-Glycosylation inte Null mutant displays poor growth and temperature-sensitive lethality ALG2 YGL065C glycolipid mannosyltransferase activity oligosaccharide-lipid intermediate assembly endoplasmic reticulum YER103W glycosyltransferase glycosyltransferase Null mutant is inviable, mutants accumulate Man1-2GlcNAc2 and arrest at G1 ALG6 YOR002W transferase activity, transferring hexosyl groups protein amino acid glycosylation* endoplasmic reticulum YDL096C YAL023C YDL095W YFL036W YGL226C-A YOR085W Required for glucosylation in the N-linked glycosylation pathway glucosyltransferase Null mutant is viable and defective in protein glycosylation. ALG7 YBR243C UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity N-linked glycosylation endoplasmic reticulum YER081W ER protein that transfers Glc-Nac-P from UDP-GlcNac to Dol-P UDP-N-acetyl-glucosamine-1-P transferase (GPT) Asparagine-linked glycosylation deficient; Null mutant is inviable ALG9 YNL219C mannosyltransferase activity protein amino acid glycosylation* endoplasmic reticulum YGR149W catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides mannosyltransferase accumulation of lipid-linked Man6GlcNAc2; hypoglycosylation of secreted proteins ARE1 YCR048W sterol O-acyltransferase activity sterol metabolism endoplasmic reticulum YCL026C-A YLR427W YLR242C Acyl-CoA cholesterol acyltransferase (sterol-ester synthetase) acyl-CoA cholesterol acyltransferase (sterol-ester synthetase) Null mutant is viable, slightly reduces in vivo and in vitro ergosterol esterification. Deletion of ARE2 YNR019W sterol O-acyltransferase activity sterol metabolism endoplasmic reticulum YDL006W YOR381W YLR242C Acyl-CoA cholesterol acyltransferase (sterol-ester synthetase) acyl-CoA cholesterol acyltransferase (sterol-ester synthetase) Null mutant is viable; greatly reduces in vivo and in vitro ergosterol esterification (to 15 - 35 % ARV1 YLR242C molecular_function unknown sterol transport* endoplasmic reticulum* YCR048W YNR019W YDL239C YHR007C YBR164C ARE2 Required for Viability 1. similar to Nup120p and C.elegans R05H5.5 protein Protein involved in sterol distribution temperature sensitive, anaerobically inviable, polyene antibiotic sensitive, inviable in the absence ASP3-1 YLR155C asparaginase activity asparagine catabolism* endoplasmic reticulum* nitrogen catabolite-regulated cell-wall L-asparaginase II nitrogen catabolite-regulated cell-wall L-asparaginase II AVT2 YEL064C transporter activity transport endoplasmic reticulum YIR034C YNR058W YOR242C YDL226C YBR103W related to the neuronal gamma -aminobutyric acid-glycine vesicular transporters transporter BIG1 YHR101C molecular_function unknown cell wall biosynthesis (sensu Fungi) endoplasmic reticulum* YMR200W YBR229C bad in glucose or big cells Null mutant is viable but shows very slow growth on glucose, cells are big and accumulate increased BSD2 YBR290W molecular_function unknown protein-vacuolar targeting* endoplasmic reticulum YNL153C YML094W metal homeostasis protein; putative membrane protein suppressor of oxygen toxicity in sod1 mutants, increased sensitivity to copper and cadmium toxicity, BST1 YFL025C molecular_function unknown ER-associated protein catabolism* endoplasmic reticulum* YLR039C YLR262C Negatively regulates COPII vesicle formation Null mutant is viable, suppresses sec13 null mutants, and shows defects in ER retention and cargo so CHO1 YER026C CDP-diacylglycerol-serine O-phosphatidyltransferase activity phosphatidylserine biosynthesis endoplasmic reticulum YMR153W phosphatidylserine synthase phosphatidylserine synthase The null mutant is viable but grows slowly on minimal medium. The growth rate of the null mutant on CHO2 YGR157W phosphatidylethanolamine N-methyltransferase activity phosphatidylcholine biosynthesis endoplasmic reticulum YKL190W YPL031C YOR070C YGR166W YBR229C First step in the methylation pathway for phosphatidylcholine biosynthesis phosphatidyl-ethanolamine N-methyltransferase Null mutant is viable and accumulates phosphatidylethanolamine and has reduced levels of phosphatidy CPT1 YNL130C diacylglycerol cholinephosphotransferase activity phosphatidylcholine biosynthesis endoplasmic reticulum Phospholipid biosynthesis sn-1,2-diacylglycerol cholinephosphotransferase Null mutant is viable, cpt1 ept1 double deletion mutants are viable CTS1 YLR286C chitinase activity cytokinesis, completion of separation endoplasmic reticulum* YNL329C Endochitinase endochitinase Null mutant is viable; exhibits a defect in cell separation DPM1 YPR183W transferase activity, transferring glycosyl groups* N-linked glycosylation* endoplasmic reticulum* YDL226C YDR311W YOR285W YER179W YFR028C dolichol phosphate mannose synthase dolichol phosphate mannose synthase Null mutant is inviable DSL1 YNL258C molecular_function unknown retrograde (Golgi to ER) transport endoplasmic reticulum YKR022C YLR440C YBL052C YFL021W YHR056C YLR457C YGL145W dsl1 mutations are suppressed by a dominant allele of SLY1, called sly1-20 EMP24 YGL200C molecular_function unknown ER to Golgi transport* endoplasmic reticulum* YML012W YAR002C-A YAL007C YDL147W YGL058W YNL192W YBR023C YLR330W YMR307W YDR420W YLR113W YPR159W YHR181W YLR057W YGL027C type I transmembrane protein, component of COPII-coated, ER-derived transport vesicles type I transmembrane protein Null mutant is viable EPT1 YHR123W ethanolaminephosphotransferase activity phosphatidylethanolamine biosynthesis endoplasmic reticulum YIR038C YEL017W YBR038W sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase Null mutant is viable ERG1 YGR175C squalene monooxygenase activity ergosterol biosynthesis endoplasmic reticulum* YNL311C squalene epoxidase; an essential enzyme in the ergosterol-biosynthesis pathway; catalyzes the epoxid squalene monooxygenase Null mutant is inviable when cells are grown under aerobic conditions; erg1 null mutants are viable ERG11 YHR007C sterol 14-demethylase activity ergosterol biosynthesis endoplasmic reticulum YHR045W YPR090W YBL007C YLR242C YHR030C YEL031W YER083C YER155C YDR310C YHR012W YKR001C YGL020C YOR070C YDL074C YLR015W YMR272C YBR121C YNL231C YJR073C YDL155W YOR275C YML013W YER031C YBR283C YMR022W YMR264W YPL170W cytochrome P450 lanosterol 14a-demethylase Null mutant is inviable, erg11 null inviability is suppressed by deletion of ERG3; erg11 mutants are ERG2 YMR202W C-8 sterol isomerase activity ergosterol biosynthesis endoplasmic reticulum YGL127C YJR091C YCR034W YNL271C sterol biosynthesis C-8 sterol isomerase synthetic lethal with vma2. ERG24 YNL280C C-14 sterol reductase activity ergosterol biosynthesis endoplasmic reticulum YDL153C YHR114W sterol C-14 reductase sterol C-14 reductase Null mutant appears to be inviable in some genetic backgrounds and conditionally lethal in others; e ERG26 YGL001C C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity ergosterol biosynthesis endoplasmic reticulum* YGR060W YBR202W one of three enzymatic activities required for the conversion of 4,4-dimethylzymosterol to zymostero C-3 sterol dehydrogenase ERG27 YLR100W 3-keto sterol reductase activity ergosterol biosynthesis endoplasmic reticulum* YGL137W YDL132W YBL005W 3-keto sterol reductase 3-keto sterol reductase ERG3 YLR056W C-5 sterol desaturase activity ergosterol biosynthesis endoplasmic reticulum YHR128W YIL074C YOL099C YOR167C YNL271C C-5 sterol desaturase C-5 sterol desaturase Null mutant is inviable; suppresses syringomycin resistant mutant; sensitive to photoactivated 3-car ERG4 YGL012W delta24(24-1) sterol reductase activity ergosterol biosynthesis endoplasmic reticulum Sterol C-24 reductase sterol C-24 reductase Null mutant is viable ERG5 YMR015C C-22 sterol desaturase activity ergosterol biosynthesis endoplasmic reticulum cytochrome P450 involved in C-22 denaturation of the ergosterol side-chain cytochrome P450|involved in C-22 denaturation of the ergosterol side-chain Null mutant is viable ERG6 YML008C sterol 24-C-methyltransferase activity ergosterol biosynthesis endoplasmic reticulum* YCL023C YDL089W YHR114W YOR097C YPL020C YPR113W YMR059W ergosterol synthesis The null mutant is viable, cannot methylate ergosterol precursors at C-24, and lacks ergosterol. The ERG9 YHR190W farnesyl-diphosphate farnesyltransferase activity ergosterol biosynthesis endoplasmic reticulum YIR038C YMR153W YLR453C squalene synthetase squalene synthetase Null mutant is inviable ERI1 YPL096C-A GTPase inhibitor activity small GTPase mediated signal transduction endoplasmic reticulum ERO1 YML130C electron carrier activity protein folding* endoplasmic reticulum YLR342W YLR208W YML067C YAL042W YDR021W YJL002C YCR057C essential, FAD-dependent oxidase of protein disulfide isomerase Null mutant is inviable; in ero1-1(ts) mutants newly synthesized carboxypeptidase Y is retained in t EUG1 YDR518W protein disulfide isomerase activity protein folding endoplasmic reticulum YCL043C ER protein functionally likely involved in interacting with nascent polypeptides in the ER protein disulfide isomerase homolog Null mutant is viable FEN1 YCR034W fatty acid elongase activity sphingolipid biosynthesis* endoplasmic reticulum* YMR202W YLR372W YOR049C YCR009C YDL017W YLR342W Elongase II elongates palmitoyl-CoA and stearoyl-CoA up to C22 fatty acids, and is also involved in 1,3-beta-glucan synthase subunit (putative) ELO1 homolog Null mutant is viable; slow growth; fenpropimorph resistant; resistant to a pneumocandin B0 analog ( GPI11 YDR302W phosphoethanolamine N-methyltransferase activity GPI anchor biosynthesis endoplasmic reticulum Glycosylphosphatidylinositol (GPI) assembly GPI13 YLL031C phosphoethanolamine N-methyltransferase activity GPI anchor biosynthesis endoplasmic reticulum YHL015W Glycosylphosphatidylinositol (GPI) biosynthesis Null mutant is inviable; Gpi13p-depleted strains accumulate a GPI precursor whose glycan headgroup c GTT1 YIR038C glutathione transferase activity glutathione metabolism endoplasmic reticulum YIR038C YLL060C YCL025C YDL089W YDR120C YDR371W YEL017W YER022W YGL225W YGR121C YGR149W YGR191W YHR123W YHR190W YIL023C YJL097W YLR026C YLR088W YML048W YMR119W YNL234W YOL065C YPL020C Glutathione Transferase glutathione transferase Null mutant is viable, heat shock sensitive at stationary phase HRD3 YLR207W ubiquitin-protein ligase activity ER-associated protein catabolism endoplasmic reticulum YPL022W HMG-CoA Reductase Degradation--the HRD complex is responsible for the endoplasmic reticulum (ER)-ass Null mutant is viable, slows degradation of Hmg2p INP54 YOL065C inositol-polyphosphate 5-phosphatase activity exocytosis endoplasmic reticulum YIR038C YMR153W YJL117W YDL204W YJR010C-A INositol polyphosphate 5-Phosphatase, fourth one identified; has homology to Type I mammalian inosit inositol polyphosphate 5-phosphatase JEM1 YJL073W co-chaperone activity protein folding* endoplasmic reticulum* DnaJ-like protein of the endoplasmic reticulum membrane Null mutant is viable but has karyogamy defect; jem1 scj1 double mutant is temperature sensitive KAR2 YJL034W chaperone activity* SRP-dependent cotranslational membrane targeting, translocation* endoplasmic reticulum lumen YKL073W YOR254C YJR091C YGR032W Involved in translocation of nascent polypeptides across the ER membrane HSP70 family|mammalian BiP (GPR78) homolog null mutants are inviable; other mutants block karyogamy (nuclear fusion) during mating KRE5 YOR336W UDP-glucose:glycoprotein glucosyltransferase activity beta-1,6 glucan biosynthesis endoplasmic reticulum YER022W appears to function early in (1,6)-beta-D-glucan synthesis pathway Null mutant is viable but has aberrant morphology, reduced levels of cell wall (1,6)-beta-glucan, an LAC1 YKL008C protein transporter activity ceramide biosynthesis* endoplasmic reticulum YMR298W YHL003C YNL107W YML064C Longevity-assurance gene 1 cognate (LAG1 cognate) LAG1 longevity gene homolog Null mutant is viable but exhibits synthetic lethality with mutations in lag1. LAG1 YHL003C protein transporter activity replicative cell aging* endoplasmic reticulum YKL008C YMR047C YMR298W YKL008C Null mutant is viable LCB3 YJL134W sphingosine-1-phosphate phosphatase activity sphingolipid biosynthesis* endoplasmic reticulum YGL053W Protein involved in incorporation of exogenous long chain bases in sphingolipids dihydrosphingosine-1-phosphate phophatase Null mutant is viable, has reduced rate of exogenous long chain base incorporation into sphingolipid LCB4 YOR171C D-erythro-sphingosine kinase activity sphingolipid metabolism* endoplasmic reticulum* YER071C YOR185C YOR034C YHR178W YPL022W involved in sphingolipid biosynthesis sphingoid long chain base (LCB) kinase Null mutant is viable, exhibts 2-3% of wild-type LCB kinase activity; lcb4 is an extragenic suppress LHS1 YKL073W chaperone activity protein transport* endoplasmic reticulum lumen YHR030C YOL087C YDR394W YJL034W Lumen HSP Seventy
Required for efficient translocation of protein precursors across the ER membr Hsp70 family LRO1 YNR008W phospholipid:diacylglycerol acyltransferase activity triacylglycerol biosynthesis* endoplasmic reticulum YMR059W Lecithin cholesterol acyl transferase (LCAT) Related Orf phospholipid:diacylglycerol acyltransferase
E.C. 2.3.1.158 Null mutant is viable MNL1 YHR204W carbohydrate binding ER-associated protein catabolism endoplasmic reticulum YER126C YGL030W YOR312C YJL053W YMR304W YHR019C mannosidase like MNS1 YJR131W mannosyl-oligosaccharide 1,2-alpha-mannosidase activity N-linked glycosylation endoplasmic reticulum YGL019W YIL046W YML064C YDR235W specific alpha-mannosidase alpha-mannosidase Null mutant is viable MPD2 YOL088C protein disulfide isomerase activity protein folding endoplasmic reticulum YLR312C YHR091C protein disulfide isomerase related protein protein disulfide isomerase related protein Null mutant is viable; overproduction of Mpd2p suppresses lethality and carboxypeptidase Y maturatio MST27 YGL051W protein binding vesicle organization and biogenesis endoplasmic reticulum* YAR033W Hypothetical ORF protein with COPI and COPII bindng motifs Null: viable. Other phenotypes: - MST28 YAR033W protein binding vesicle organization and biogenesis endoplasmic reticulum* YAR033W YKL174C YGL051W Multicopy suppressor of Sec Twenty one Null: viable. Other phenotypes: - NPL4 YBR170C protein binding* mRNA-nucleus export* endoplasmic reticulum* YGR048W YDR259C YLR044C YDL126C YER081W YDL190C Nuclear pore or nuclear pore-associated protein required for nuclear membrane integrity and nuclear Temperature-sensitive mutants accumulate nuclear-targeted proteins in the cytoplasm and poly(A)+RNA OPI3 YJR073C phosphatidyl-N-methylethanolamine N-methyltransferase activity phosphatidylcholine biosynthesis endoplasmic reticulum YJL047C YKL190W YHR007C YPL031C YLR039C YLR262C YBR023C YHR142W YMR307W YBL061C YNL322C YAL013W Second and third steps of methylation pathway for phosphatidylcholine biosynthesis unsaturated phospholipid N-methyltransferase Null mutant is viable, temperature sensitive in the presence of monomethylethanolamine, exhibits an OSH1 YAR044W oxysterol binding* steroid biosynthesis endoplasmic reticulum* YLR397C YGR192C YER120W May be involved in ergosterol synthesis Null mutant is viable but displays pleiotropic sterol-related phenotypes such as tryptophan-transpor OST1 YJL002C dolichyl-diphosphooligosaccharide-protein glycosyltransferase activity N-linked glycosylation* endoplasmic reticulum lumen* YLR208W YGL100W YMR146C YML130C YML019W YGL022W YEL002C YDR034C Oligosaccharyltransferase catalyzes the transfer of oligosaccharide from dolichol-oligosaccharide do 64 kDa, alpha subunit of oligosaccharyltransferase complex; homologous to mammalian ribophorin I Null mutant is inviable; temperature-sensitive mutants show pleiotropic underglycosylation of solubl PBN1 YCL052C molecular_function unknown protein processing endoplasmic reticulum YEL060C Protease B Non-derepressible protease B nonderepressible form Null mutant is inviable; overexpression of both PBN1 and LRE1 confers resistance to laminarinase, wh PDI1 YCL043C protein disulfide isomerase activity protein folding endoplasmic reticulum lumen YER189W YLR354C YDR518W YJR076C YIR001C YLR233C YIL035C YOL126C Catalyzes the formation and isomerization of disulfide bonds during the folding of secretory protein protein disulfide isomerase Null mutant is inviable PHO86 YJL117W molecular_function unknown secretory pathway* endoplasmic reticulum YLR423C YMR153W YBR101C YHR114W YDL145C YDL116W YMR106C YOL065C YGR229C May collaborate with Pho87p and Pho84p in phosphate uptake inorganic phosphate transporter (putative) Null mutant is viable and expresses repressible acid phosphatase in high phosphate medium; pho86 pho PIS1 YPR113W CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity phosphatidylinositol biosynthesis endoplasmic reticulum YML008C YDL226C phosphatidylinositol synthase phosphatidylinositol synthase Null mutant is inviable PMT2 YAL023C dolichyl-phosphate-mannose-protein mannosyltransferase activity O-linked glycosylation endoplasmic reticulum YKL190W YLR039C YOR002W Transfers mannosyl residues from dolichyl phosphate-D-mannose to seryl and threonyl residues in prot dolichyl phosphate-D-mannose:protein O-D-mannosyltransferase Null mutants are viable but show diminished in vitro and in vivo O-mannosylation activity; pmt1 pmt2 PMT3 YOR321W dolichyl-phosphate-mannose-protein mannosyltransferase activity O-linked glycosylation endoplasmic reticulum YDL153C Transfers mannose residues from dolichyl phosphate-D-mannose to specific serine/threonine residues o dolichyl phosphate-D-mannose:protein O-D-mannosyltransferase Null mutant is viable; pmt2 pmt3 pmt4 triple mutant is inviable PMT4 YJR143C dolichyl-phosphate-mannose-protein mannosyltransferase activity O-linked glycosylation endoplasmic reticulum Transfers mannose residues from dolichyl phosphate-D-mannose to specific serine/threonine residues o dolichyl phosphate-D-mannose:protein O-D-mannosyltransferase Null mutant is viable but shows under glycosylation of chitinase; pmt2 pmt3 pmt4 triple mutant is in PMT5 YDL093W dolichyl-phosphate-mannose-protein mannosyltransferase activity O-linked glycosylation endoplasmic reticulum YGL055W Transfers mannose residues from dolichyl phosphate-D-mannose to specific serine/threonine residues o dolichyl phosphate-D-mannose:protein O-D-mannosyltransferase PMT6 YGR199W dolichyl-phosphate-mannose-protein mannosyltransferase activity O-linked glycosylation endoplasmic reticulum YBR059C Transfers mannose residues from dolichyl phosphate-D-mannose to specific serine/threonine residues o dolichyl phosphate-D-mannose:protein O-D-mannosyltransferase PRM8 YGL053W molecular_function unknown conjugation with cellular fusion endoplasmic reticulum* YJL134W YLR065C YOL107W YOR059C YOR245C YOR307C pheromone-regulated membrane protein PRM9 YAR031W molecular_function unknown conjugation with cellular fusion endoplasmic reticulum* YBR217W YCR030C YJR091C pheromone-regulated membrane protein PRY1 YJL079C molecular_function unknown biological_process unknown endoplasmic reticulum* YPR086W Pathogen Related in Sc, contains homology to the plant PR-1 class of pathogen related proteins. The QRI8 YMR022W ubiquitin-protein ligase activity* ER-associated protein catabolism endoplasmic reticulum YLL039C YIL033C YNR031C YLR006C YLR109W YER100W YHR007C part of the HRDDER pathway of ER-associated protein degradation ubiquitin-conjugating enzyme Overexpression confers resistance to methylmercury. RER2 YBR002C prenyltransferase activity protein amino acid glycosylation* endoplasmic reticulum cis-prenyltransferase involved in dolichol synthesis cis-prenyltransferase Null mutant is viable but both heat and cold sensitive and mislocalizes several ER proteins. rer2-1 RHK1 YBL082C alpha-1,3-mannosyltransferase activity protein amino acid glycosylation* endoplasmic reticulum YEL002C YKL210W YNL322C Resistance to Hansenula Killer 1, hypothetical F-458 protein Dol-P-Man dependent alpha(1-3) mannosyltransferase (putative) Null mutant is viable, resistant to Hansenula killer toxin ROT2 YBR229C alpha-glucosidase activity cell wall biosynthesis (sensu Fungi) endoplasmic reticulum YFL036W YJL095W YLR087C YOR068C YOR069W YOR070C YDR389W YGL084C YBR221C YGR157W YKL203C YHR108W YHR101C YHR030C YMR307W YNL322C Reversal of tor2 lethality. Involved in Beta-1,6-glucan synthesis. glucosidase II Null mutant is inviable; rot2 mutations can suppress tor2 mutations; synthetically lethal with rot1 SBH1 YER087C-B protein transporter activity cotranslational membrane targeting translocon YJL091C YLR262C Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein transl Sbh2p homolog Null mutant is viable. sbh1 sbh2 double deletion mutants exhibit synthetic temperature sensitivity a SBH2 YER019C-A protein transporter activity cotranslational membrane targeting endoplasmic reticulum* YJR091C YDR074W Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulu Sbh1p homolog Null mutant is viable. sbh1 sbh2 double deletion mutants exhibit synthetic temperature sensitivity a SCJ1 YMR214W co-chaperone activity protein folding endoplasmic reticulum lumen YBR162C YGL027C dnaJ homolog DnaJ homolog Null mutant is viable but exhibits defects in protein sorting and sensitivity to tunicamycin. SCS2 YER120W protein binding protein-ER targeting* endoplasmic reticulum* YLR305C YHR200W YLR342W YDR150W YGR086C YAR042W YHL020C YAR044W YDL019C YMR049C YHR196W YNL027W YLR039C YLR262C Suppressor of Choline Synthesis
Likely to be involved in regulating INO1 expression, suppressor o Null mutant is viable but is an inositol auxotroph above 34 deg. SCS7 YMR272C oxidoreductase activity fatty acid metabolism endoplasmic reticulum YPL057C YBR036C YHR007C YOR070C YLR039C YLR103C YLR113W Required for the hydroxylation of the very long chain fatty acid (VLCFA), located in the endoplasmic desaturase|hydroxylase Null mutant is viable, suppresses the Ca2+-sensitive phenotype of csg2 delta mutants SEC11 YIR022W signal peptidase activity signal peptide processing endoplasmic reticulum* YPR163C signal peptidase subunit Null mutant is inviable SEC20 YDR498C v-SNARE activity nonselective vesicle fusion* endoplasmic reticulum* YLR268W YGL145W YOR075W Identified in a screen for dense cells that accumulated invertase at the non-permissive temperature, v-SNARE secretion deficient SEC22 YLR268W v-SNARE activity ER to Golgi transport* endoplasmic reticulum* YML077W YLR026C YPR181C YDR004W YGL145W YIL109C YLR078C YDR498C YOR075W YHR030C YKR068C YER157W YPL218W YBR234C YJL168C YOR070C YLR418C YLR085C YPR135W YLR103C YDR217C YJR057W YLR330W YBL061C YCR009C YDR388W YAL013W YMR263W Identified in a screen for dense cells that accumulated invertase at the non-permissive temperature, null mutant is cold and heat sensitive. Defective in ER to Golgi transport. SEC65 YML105C molecular_function unknown SRP-dependent cotranslational membrane targeting, signal sequence recognition signal recognition particle YBL032W YPR088C YPR086W signal recognition particle subunit, homologue of mammalian SRP19 Null mutant is viable, temperature sensitive SED4 YCR067C molecular_function unknown ER to Golgi transport endoplasmic reticulum YNL171C YOR128C Sed4p is an integral ER membrane protein, which, along along with its close homolog, Sec12p, is invo Null mutant is viable, shows decreased rate of ER to Golgi transport SFB3 YHR098C molecular_function unknown ER to Golgi transport endoplasmic reticulum* YPR181C YJL199C YJR048W YPL204W YLR208W YBL106C binds to Sed5p and Sec23p by distinct domains
Lethal with sec-thirteen similar to SEC24 SIL1 YOL031C molecular_function unknown SRP-dependent cotranslational membrane targeting, translocation endoplasmic reticulum YLR412W ER-localized protein required for protein translocation into the ER, interacts with the ATPase domai Nucleotide exchange factor SLY1 YDR189W SNARE binding ER to Golgi transport endoplasmic reticulum* YKR068C YKL196C YBL050W YOR075W YMR316W YNL239W YLR026C YER157W Hydrophilic suppressor of ypt1 involved in vesicle trafficking between ER and Golgi
Sm like prote t-SNARE-interacting protein that functions in ER-to-Golgi traffic Null mutant is inviable; SLY1-20, which differs from wild-type SLY1 by a single amino acid, is a sin SOP4 YJL192C molecular_function unknown ER to Golgi transport endoplasmic reticulum* YJR091C suppressor of pma1-7 SPC1 YJR010C-A molecular_function unknown signal peptide processing signal peptidase complex YJR091C YLR447C YOL065C Homolog of the SPC12 subunit of mammalian signal peptidase complex. Protein is important for efficie Null mutant is viable; synthetically lethal with a conditional mutation in sec11; high copy Spc1 sup SPC2 YML055W protein binding signal peptide processing signal peptidase complex YBR217W YOL016C YER022W YLR078C subunit of signal peptidase complex, homologous to mammalian protein SPC25 signal peptidase complex subunit|similar to mammalian protein SPC25 Null mutant is viable. spc1 spc2 double deletion mutants grow relatively well as compared to wild-ty SPC3 YLR066W signal peptidase activity signal peptide processing signal peptidase complex YJR091C signal peptidase subunit Null mutant is inviable. SPT14 YPL175W transferase activity, transferring hexosyl groups GPI anchor biosynthesis endoplasmic reticulum YDR215C N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein suppression of Ty transcription SRP14 YDL092W signal sequence binding protein-ER targeting signal recognition particle YPR088C YKL122C Signal recognition particle subunit Null mutant is viable SRP54 YPR088C signal sequence binding protein-ER targeting signal recognition particle YCL037C YOR128C YER155C YDL051W YDL092W YML105C YKL122C YPL243W YPL210C YOR272W YKR036C YMR106C Signal recognition particle subunit (homolog of mammalian SRP54) Amaya et al. (J. Biochem. (Tokyo) 107:457-463) report that a SRP54 deletion is inviable, whereas Han SRP68 YPL243W signal sequence binding protein-ER targeting signal recognition particle YPR088C part of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in prot signal recognition particle component Null mutant is viable, associated with slow cell growth and inefficient protein translocation across SRP72 YPL210C signal sequence binding protein-ER targeting signal recognition particle YPR088C part of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in prot signal recognition particle component Null mutant is viable, associated with slow cell growth and inefficient protein translocation across SSS1 YDR086C protein transporter activity cotranslational membrane targeting translocon YLR378C YBL022C Subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p), involved in transfer of secreto ER protein|Sec61 trimeric complex component|Ssh1 trimeric complex component Null mutant is inviable. Depletion of the Sss1 protein rapidly results in accumulation of multiple s SUR2 YDR297W sphingosine hydroxylase activity sphingolipid biosynthesis* endoplasmic reticulum YCR009C YBR036C Possibly hydroxylates ceramide-1 at C-4 to give ceramide-2. Or, hydroxylates DHS at C-4 to give PHS, sphingosine hydroxylase Null mutant has altered phospholipid levels; suppressor of rvs161 and rvs167 mutations. SUR4 YLR372W fatty acid elongase activity sphingolipid biosynthesis* endoplasmic reticulum* YPL076W YCR034W YCR009C YPL031C Elongase III synthesizes 20-26-carbon fatty acids from C18-CoA primers elongase Null mutants is viable, not sensitive to UV or gamma radiation. sur4 mutants suppress rad3, rvs161 d TIP20 YGL145W molecular_function unknown retrograde (Golgi to ER) transport endoplasmic reticulum YDR498C YOR075W YNL258C YLR373C YPR086W YLR268W YPR105C transport protein that interacts with Sec20p; required for protein transport from the endoplasmic re transport protein that interacts with Sec20p; required for protein transport from the endoplasmic re UFD1 YGR048W protein binding ubiquitin-dependent protein catabolism* endoplasmic reticulum YHR039C-A YBR170C YDL126C YKR002W YDR390C YPL222W Ubiquitin fusion degradation protein Homozygous ufd1-1 mutant diploids exhibit sporulation defects. loss of Ufd1 blocks ER-associated pro USE1 YGL098W SNAP receptor activity retrograde (Golgi to ER) transport endoplasmic reticulum YEL017W YDR373W Use1p is a SNARE and forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p. The protein is locali VMA21 YGR105W molecular_function unknown protein complex assembly endoplasmic reticulum* YFR018C YKL190W YLR039C YLR262C YPR159W YBR164C YAL013W YDR126W Protein involved in vacuolar H-ATPase assembly or function. Required for the biogenesis of a functio Null mutant is viable but grows slowly and exhibits increased calcium sensitivity. Null mutants also WBP1 YEL002C dolichyl-diphosphooligosaccharide-protein glycosyltransferase activity N-linked glycosylation* endoplasmic reticulum* YPL227C YGL022W YMR149W YJL002C YML115C YBL082C wheat germ agglutinin-binding protein oligosaccharyl transferase glycoprotein complex, beta subunit lethal YDC1 YPL087W ceramidase activity response to heat* endoplasmic reticulum Yeast dihydro-ceramidase alkaline dihydroceramidase with minor reverse activity Null mutant is viable. YEA4 YEL004W UDP-N-acetylglucosamine transporter activity cell wall chitin biosynthesis* endoplasmic reticulum Shows sequence similarity to GOG5, a gene involved in vanadate resistance YOS9 YDR057W protein transporter activity ER to Golgi transport endoplasmic reticulum Appears to play a direct role in the transport of GPI-anchored proteins to the Golgi apparatus. membrane-associated glycoprotein Accelerates Gas1 transport and processing in cells overexpressing YOS9. Gas1 processing is slowed in YPC1 YBR183W ceramidase activity ceramide metabolism endoplasmic reticulum Yeast Phyto-ceramidase alkaline ceramidase with reverse activity Null mutant is viable and two times more heat resistant than the wild-type parental strain. YSR3 YKR053C sphingosine-1-phosphate phosphatase activity sphingolipid biosynthesis endoplasmic reticulum YDR403W Yeast Sphingolipid Resistance Gene DHS-1-P phosphatase Null mutant is viable and accumulates of dihydrosphingosine-1-P YBR096W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YBR159W ketoreductase activity fatty acid elongation endoplasmic reticulum* microsomal beta-keto-reductase YBR273C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YBR287W molecular_function unknown biological_process unknown endoplasmic reticulum YCL045C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YDL072C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YDL121C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YDL193W prenyltransferase activity biological_process unknown endoplasmic reticulum* Protein required for cell viability YDR056C molecular_function unknown biological_process unknown endoplasmic reticulum* Hypothetical ORF YDR196C dephospho-CoA kinase activity coenzyme A biosynthesis endoplasmic reticulum* predicted to catalyze the final step in synthesis of Coenzyme A YDR221W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YDR307W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YDR411C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YDR437W molecular_function unknown biological_process unknown endoplasmic reticulum* Protein required for cell viability YDR476C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YDR492W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YEL001C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YEL043W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YER004W molecular_function unknown biological_process unknown endoplasmic reticulum The authentic, non-tagged protein was localized to the mitochondria YER053C-A molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YFR041C molecular_function unknown biological_process unknown endoplasmic reticulum Endoplasmic reticulum protein that may function as a cochaperone, as suggested by the presence of a YGL010W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YGL231C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YGR263C molecular_function unknown biological_process unknown endoplasmic reticulum presents weak similarity to a putative E. coli protein defined as a lipase-like enzyme YHR036W molecular_function unknown biological_process unknown endoplasmic reticulum Protein required for cell viability YHR045W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YIL039W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YIL090W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YJL012C-A molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF identified by homology. See FEBS Letters [2000] 487:31-36. YJL097W molecular_function unknown biological_process unknown endoplasmic reticulum* Protein required for cell viability YJR088C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YLL014W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YLL023C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YLL055W ion transporter activity biological_process unknown endoplasmic reticulum Hypothetical ORF YLR023C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YLR050C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YLR064W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YLR440C molecular_function unknown biological_process unknown endoplasmic reticulum* Protein required for cell viability YML059C hydrolase activity biological_process unknown endoplasmic reticulum Hypothetical ORF YML125C molecular_function unknown biological_process unknown endoplasmic reticulum Protein required for cell viability YMR298W molecular_function unknown biological_process unknown endoplasmic reticulum* Protein required for cell viability YNL046W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YNL146W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YNL149C molecular_function unknown biological_process unknown endoplasmic reticulum* Protein required for cell viability YNL181W oxidoreductase activity* biological_process unknown endoplasmic reticulum* Protein required for cell viability YNR021W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YOR044W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YOR175C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YOR285W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YPL207W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YPR003C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YPR063C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YPR091C molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF YPR114W molecular_function unknown biological_process unknown endoplasmic reticulum Hypothetical ORF